Neurobiol Aging 2012 Jan;33 (1): 176-86. [IF:6.634]
Luteolin from Purple Perilla mitigates ROS insult particularly in primary neurons.
Zhao G , Yao-Yue C , Qin GW , Guo LH .
Cell Star Bio-Technologies, Co., Limited, Shanghai, People's Republic of China; Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, People's Republic of China.
中国科学院上海生命科学研究院生物化学与细胞生物学研究所
Abstract
Increased attention has been paid to the role of oxidant/antioxidant imbalance in neurodegenerative process and pharmaceutical neuroprotective interventions. Food-derived compound luteolin possesses multitarget actions including reactive oxygen species (ROS)-scavenging activity in cultured human endothelial cells or permanent immature rat oligodendrocytes. This study aims to elucidate whether luteolin has a neuroprotective tendency toward ROS-insulted neural cells. The present results showed that luteolin, isolated from the ripe seed of Perilla frutescens (L.) Britt., markedly reversed hydrogen peroxide-induced cytotoxicity in primary culture cortical neurons but not in cultured human neuroblastoma cells. Upon the ROS-insulted primary neurons, luteolin concentration-dependently enhanced neuronal cell survival with efficacy higher than and potency similar to vitamin E. Additionally, luteolin significantly attenuated the increase in ROS production and prevented the decreases in activities of mitochondria, catalase, and glutathione in ROS-insulted primary neurons. Thus, luteolin functions by neuroprotection possibly through a rebalancing of pro-oxidant-antioxidant status. This agent points to possible interventions for preventing neurodegenerative diseases such as cerebral ischemia, Parkinson's disease, and Alzheimer's disease, as well as for improving brain aging.
摘要：
在神经变性过程和药物的神经保护性干预中，氧化与抗氧化失衡的作用越来越受到关注。
食物衍生的化合物木犀草素具有多重靶向作用，包括在体外培养的人内皮细胞或者恒定未成熟的鼠少突神经胶质细胞中清除活性氧（ROS）的活性。
这项研究旨在阐述木犀草素能否对活性氧损伤的神经细胞具有神经保护性趋势。
目前的结果显示，在初级培养的皮质神经元中，从成熟紫苏种子中分离出来的木犀草素能明显逆转过氧化氢诱导的细胞毒性，但在培养的人神经母细胞瘤细胞中无此作用。
在活性氧损伤的初级神经元中，浓度依赖的木犀草素提高了神经细胞的存活，其能力与维生素类似，但更高效。
另外，在活性氧损伤的初级神经元中，木犀草素明显减缓了活性氧的生成，并且阻止了线粒体，过氧化氢酶和谷胱甘肽活性的下降。
所以，木犀草素神经保护作用可能通过氧化和抗氧化的再平衡。这种物质意味着可能阻止神经变性疾病的干预，如脑缺血，帕金森病，和阿尔茨海默病，当然也可以用来改善大脑老化。
